Extended Data Fig. 4. Effect of LRF on thymocyte α4β7 expression.

(a) Histogram overlays show the expression of α4β7 on wild-type IELp and conventional CD8⁺ SP thymocytes cells. Gray-shaded histogram (Bkgd) shows background of PE fluorochrome signal in IELp from Ctrl mice for which the primary α4β7 antibody was omitted from the staining mix. Right graphs show protein expression (MFI), computed on cells expressing each protein (left plot bracket).

(b) Histogram (left) shows the expression of α4β7 on TCRβ^hiCD8α⁺ SP thymocytes from Ctrl or LRF KO mice, displayed as in (a). Graph (right) shows the percentage of α4β7⁺ cells among TCRβ^hi CD8α⁺ SP thymocytes.

(c) Expression of α4β7 on Ctrl (solid line) and LRF KO (dashed line) TCRβ^hiCD4⁺ SP thymocytes, displayed as in (a). (a-c) Data are from one experiment (4 mice per group) representative of two. Each symbol in summary graphs represents one mouse.

Error bars indicate standard error of the mean (SEM). P values (a, b) are from two-tailed unpaired t-test.