TABLE 3.
Comparison of B. subtilis subsp. niger measurements obtained for flooring materials with two levels of background P. chrysogenum contaminationa
| Flooring material | Background P. chrysogenum level (CFU/cm2) | Log10B. subtilis subsp. niger concn (SE)
|
|||
|---|---|---|---|---|---|
| Swab kit
|
Sponge swipe
|
||||
| PCR (templates/cm2) | Culture (CFU/cm2) | PCR (templates/cm2) | Culture (CFU/cm2) | ||
| Vinyl tile | 102 | 3.531 (0.014) | 2.842 (0.077) | 3.347 (0.073) | 2.981 (0.102) |
| Commercial carpet | 102 | 3.007 (0.001) | 2.468 (0.057) | 2.948 (0.038) | 2.506 (0.083) |
| Residential carpet | 102 | 2.755 (0.092) | 2.210 (0.071) | 2.599 (0.599) | 2.696 (0.108) |
| Soiled residential carpet | 102 | 2.587 (0.160) | 2.173 (0.139) | 2.428 (0.090) | 2.083 (0.134) |
| Vinyl tile | 104 | 3.721 (0.251) | 3.005 (0.300) | 3.555 (0.128) | 2.755 (0.392) |
| Commercial carpet | 104 | 3.119 (0.272) | 2.394 (0.260) | 3.018 (0.099) | 2.516 (0.111) |
| Residential carpet | 104 | 2.732 (0.125) | 2.079 (0.070) | 2.856 (0.033) | 2.369 (0.002) |
| Soiled residential carpet | 104 | 2.576 (0.190) | 1.897 (0.149) | 2.554 (0.086) | 2.172 (0.127) |
a
The swab kit and sponge swipe sampling methods were used to sample four flooring materials for culture and QPCR analysis (n = 2).