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Gating strategy of leukocyte subpopulation in peritoneal fluids. The cells were immunostained as described with mAbs to CD11b, CD4, CD8, and CD45 together with DAPI and FVS780 as described in Section 2. Live and nucleated cells were selected in DAPI (+) and FVS780(−) gate as described in legend of Figure 1 and ratios of positive cells to CD45(+) cells were calculated. DAPI, 4,6‐diamidino‐2‐phenylindole [Color figure can be viewed at wileyonlinelibrary.com]
