Table 3. Minimum bactericidal dilutions for various decontaminating agents at different holding times.
| Species | Strain | Holding time (min) | Minimum bactericidal dilution (median and range) | ||||
|---|---|---|---|---|---|---|---|
| Bleach | HLD4L | Prevail™ | TriGene | Virkon | |||
| Acinetobacter baumannii | BAA-1790 | 10 | 1:8 (1:8–1:16)1 | 1:1,600 | 1:40 | 1:3,200 (1:1,600–1:3,200) | 1:400 (1:200–1:400) |
| 30 | 1:16 (1:16–1:32) | 1:1,600 (1:1,600–1:3,200) | 1:40 | 1:3,200 (1:3,200–1:6,400) | 1:400 (1:200–1:400) | ||
| 60 | 1:32 (1:32–1:64) | 1:1,600 (1:1,600–1:3,200) | 1:40 | 1:3,200 (1:3,200–1:6,400) | 1:400 (1:200–1:400) | ||
| 240 | 1:64 | 1:3,200 (1:1,600–1:3,200) | 1:40 | 1:3,200 (1:3,200–1:6,400) | 1:400 | ||
| 1,440 | 1:64 | 1:1,600 (1:1,600–1:3,200) | 1:80 (1:40–1:80) | 1:6,400 | 1:400 | ||
| Bacillus spizizenii | ATCC 6633 | 10 | 1:2 | 1:3,200 | 1:640 (1:256–1:640 | 1:3,200 (1:1,600–1:3,200) | 1:40 |
| 30 | 1:2 | 1:3,200 | 1:320 (1:256–1:640) | 1:3,200 (1:1,600–1:3,200) | 1:40 | ||
| 60 | 1:2 | 1:3,200 | 1:640 (1:256–1:640) | 1:3,200 (1:1,600–1:3,200) | 1:40 (1:40–1:80) | ||
| 240 | 1:2 | 1:3,200 | 1:640 (1:256–1:640) | 1:3,200 (1:1,600–1:6,400) | 1:80 | ||
| 1,440 | 1:2 | 1:3,200 | 1:640 (1:256–1:1,280) | 1:3,200 (1:1,600–1:3,200) | 1:80 (1:80–1:160) | ||
| Escherichia coli | ATCC 25922 | 10 | 1:10 (1:10–1:16) | 1:400 (1:400–1:800) | 1:40 (1:40–1:80) | 1:1,600 (1:1,600–1:3,200) | 1:200 (1:100–1:200) |
| 30 | 1:10 (1:8–1:20) | 1:800 | 1:80 | 1:3,200 (1:1,600–1:3,200) | 1:200 (1:200–1:400) | ||
| 60 | 1:32 (1:20–1:40) | 1:800 | 1:80 | 1:3,200 | 1:200 (1:200–1:400) | ||
| 240 | 1:40 (1:40–1:64) | 1:1,600 (1:800–1:1,600) | 1:80 | 1:3,200 (1:3,200–1:6,400) | 1:200 (1:200–1:400) | ||
| 1,440 | 1:40 (1:40–1:64) | 1:3,200 | 1:80 | 1:6,400 | 1:200 (1:200–1:400) | ||
| BAA-2340 | 10 | 1:8 (1:8–1:32) | 1:400 | 1:80 (1:40–1:80) | 1:1,600 | 1:200 | |
| 30 | 1:32 | 1:400 | 1:80 (1:40–1:80) | 1:1,600 | 1:200 | ||
| 60 | 1:32 (1:32–1:64) | 1:400 (1:400–1:800) | 1:80 | 1:3,200 (1:1,600–1:3,200) | 1:200 (1:200–1:400) | ||
| 240 | 1:64 (1:32-1:64) | 1:800 | 1:80 (1:80–1:160) | 1:3,200 | 1:400 | ||
| 1,440 | 1:64 (1:32–1:64) | 1:1,600 (1:800–1:1,600) | 1:160 (1:80–1:320) | 1:3,200 (1:3,200–1:6,400) | 1:400 | ||
| BAA-2452 | 10 | 1:10 (1:10-1:20) | 1:400 | 1:20 (1:20–1:40) | 1:800 (1:800–1:1,600) | 1:80 (1:80–1:100) | |
| 30 | 1:20 (1:10–1:40) | 1:800 | 1:20 (1:20–1:40) | 1:1,600 | 1:160 (1:160–1:200) | ||
| 60 | 1:20 (1:20–1:40) | 1:800 | 1:40 (1:20–1:40) | 1:1,600 | 1:160 (1:160–1:200) | ||
| 240 | 1:60 (1:20–1:80) | 1:1,600 | 1:40 (1:40–1:80) | 1:3,200 | 1:160 (1:160–1:200) | ||
| 1,440 | 1:60 (1:20–1:80) | 1:2,400 (1:1,600–1:3,200) | 1:40 (1:40–1:80) | 1:3,200 | 1:160 (1:160-1:200) | ||
| Top 10 | 10 | 1:4 (1:4–1:8) | 1:200 | 1:80 (1:80–1:128) | 1:1,600 (1:800–1:1,600) | 1:320 (1:160–1:320) | |
| 30 | 1:8 (1:4–1:8) | 1:400 (1:200-1:400) | 1:80 (1:80–1:128) | 1:1,600 (1:1,800–1:1,600) | 1:320 (1:160–1:320) | ||
| 60 | 1:16 (1:4–1:32) | 1:400 | 1:160 (1:128–1:160) | 1:1,600 (1:1,600–1:3,200) | 1:320 (1:160–1:320) | ||
| 240 | 1:64 (1:32–1:64) | 1:800 (1:400–1:800) | 1:160 (1:128–1:160) | 1:3,200 | 1:640 (1:320–1:640) | ||
| 1,440 | 1:64 (1:32–1:64) | 1:1600 | 1:160 (1:128–1:160) | 1:6,400 (1:3,200–1:6,400) | 1:640 (1:320–1:640) | ||
| Klebsiella oxytoca | ATCC 49131 | 10 | 1:16 (1:16–1:32) | 1:200 (1:200–1:400) | 1:40 | 1:800 | 1:200 (1:100–1:200) |
| 30 | 1:32 | 1:400 (1:400–1:800) | 1:40 (1:40–1:80) | 1:1,600 (1:800–1:1,600) | 1:200 | ||
| 60 | 1:32 (1:32–1:64) | 1:800 | 1:80 | 1:1,600 | 1:200 (1:200–1:400) | ||
| 240 | 1:64 | 1:800 | 1:80 | 1:3,200 | 1:400 (1:200–1:400) | ||
| 1,440 | 1:64 | 1:1,600 | 1:80 | 1:3,200 | 1:400 (1:200–1:400) | ||
| Mycobacterium abscessus | NZRM 4048 | 10 | 1:8 (1:4–1:8) | 1:8 (1:8–1:16) | 1:8 (1:8–1:16) | 1:32 | NA 2 |
| 30 | 1:8 | 1:16 | 1:16 | 1:32 | 1:20 | ||
| 60 | 1:8 (1:8–1:16) | 1:16 | 1:16 | 1:32 | 1:20 | ||
| 240 | 1:8 (1:8–1:16) | 1:16 (1:16–1:32) | 1:16 | 1:32 | 1:40 (1:20–1:80) | ||
| 1,440 | 1:16 | 1:32 (1:16–1:128) | 1:32 | 1:32 (1:32–1:64) | 1:80 (1:80–1:320) | ||
| Mycobacterium marinum | ATCC 927 | 10 | 1:8 (1:4–1:16) | 1:40 (1:32–1:64) | 1:16 | 1:104 (1:64–1:320) | NA |
| 30 | 1:12 (1:4-1:16) | 1:52 (1:32–1:80) | 1:16 | 1:104 (1:64–1:640) | NA | ||
| 60 | 1:12 (1:8-1:16) | 1:72 (1:32–1:80) | 1:16 | 1:144 (1:128–1:640) | NA | ||
| 240 | 1:16 (1:8–1:16) | 1:104 (1:64–1:160) | 1:24 (1:16–1:32) | 1:208 (1:128–1:210) | NA | ||
| 1,440 | 1:16 (1:16-1:32) | 1:288 (1:256–1:640) | 1:32 | 1:288 (1:256–1:640) | 1:80 (1:80–1:160) | ||
| Mycobacterium smegmatis | ATCC 700084 | 10 | 1:16 (1:4-1:32) | 1:400 | 1:16 | 1:800 (1:800–1:1,600) | 1:40 (1:20–1:160) |
| 30 | 1:16 (1:8–132) | 1:400 (1:400–1:800) | 1:24 (1:16–1:32) | 1:800 (1:800–1:1,600) | 1:40 (1:20–1:320) | ||
| 60 | 1:16 (1:16–1:32) | 1:800 (1:400–1:800) | 1:32 (1:32–1:64) | 1:1600 (1:800–1:1,600) | 1:40 (1:20–1:320) | ||
| 240 | 1:32 (1:32–1:64) | 1:800 | 1:128 (1:64–1:160) | 1:1,600 (1:1,600–1:3,200) | 1:80 (1:40–1:640) | ||
| 1,440 | 1:32 (1:32–1:64) | 1:1,600 | 1:128 (1:128–1:160) | 1:4,800 (1:3,200–1:6,400) | 1:160 (1:80–1:640) | ||
| Mycobacterium tuberculosis | ATCC 25618 | 10 | 1:2 (1:2–1:4) | 1:128 (1:64–1:128) | 1:16 | 1:128 | NA |
| 30 | 1:2 (1:2–1:4) | 1:64 (1:64–1:128) | 1:16 | 1:128 | NA | ||
| 60 | 1:2 (1:2–1:4) | 1:64 (1:64–1:128) | 1:16 | 1:128 | NA | ||
| 240 | 1:4 (1:2-1:4) | 1:64 (1:64–1:128) | 1:16 (1:16–1:32) | 1:128 | 1:40 | ||
| 1,440 | 1:4 (1:4–1:8) | 1:128 | 1:32 | 1:128 | 1:80 | ||
| Pseudomonas aeruginosa | ATCC 27853 | 10 | 1:16 (1:8–1:16) | 1:400 | 1:40 | 1:400 | 1:200 |
| 30 | 1:16 | 1:400 (1:400–1:800) | 1:40 | 1:400 (1:400–1:800) | 1:200 | ||
| 60 | 1:32 (1:16–1:32) | 1:400 (1:400–1:800) | 1:40 | 1:800 | 1:400 (1:200–1:400) | ||
| 240 | 1:32 | 1:400 (1:400–1:800) | 1:40 | 1:800 | 1:400 | ||
| 1,440 | 1:32 | 1:800 (1:400–1:800) | 1:40 (1:40–1:80) | 1:800 (1:800–1:1,600) | 1:400 | ||
| Staphylococcus aureus | ATCC 6538 | 10 | 1:8 (1:8–1:16) | 1:1,600 | 1:160 (1:40–1:160) | 1:3,200 (1:1,600–1:3,200) | 1:400 (1:400–1:800) |
| 30 | 1:16 (1:16–1:32) | 1:1,600 (1:1,600–1:3,200) | 1:80 (1:40–1:160) | 1:3,200 | 1:400 (1:400–1:800) | ||
| 60 | 1:16 (1:10–1:32) | 1:3,200 | 1:160 (1:80–1:320) | 1:3,200 (1:3,200–1:6,400) | 1:800 (1:400–1:1,600) | ||
| 240 | 1:32 (1:20–1:64) | 1:3,200 | 1:160 (1:80–1:640) | 1:6,400 (1:3,200–1:6,400) | 1:800 (1:400–1:1,600) | ||
| 1,440 | 1:64 (1:20–1:128) | 1:6,400 (1:3,200–1:6,400) | 1:320 (1:80–1:1,280) | 1:6,400 (1:6,400–1:12,800) | 1:1,600 (1:400–1:3,200) | ||
Notes:
1
Values shown in bold indicate a condition under which the recommended dilution would not be sufficient for decontamination.
2
Not applicable.