FIGURE 5.

Influence of Endo180 knockdown on ROS generation and of collagen cleavage on collagen remodeling in fibroblast‐embedded collagen gel. The cells were embedded into 3D collagen gel 3 days after control or Endo180 siRNA transfection and incubated for 2 days. The gels were stained with CellROX^® Oxidative Stress Reagents to visualize ROS (green), and the nuclei were counterstained with Hoechst33342 (blue). Representative images were taken using a fluorescent microscope with an axial optical sectioning module. Scale bar: 20 μm [Colour figure can be viewed at wileyonlinelibrary.com]