Table 2.
Genome detection and serology test results on clinical specimens.
| (A) | VSBV-1 RT-qPCR | BoDV-1 RT-qPCR | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Animal ID | #1 (i.c.) | #2(i.c.) | #3(i.c.) | #4 (non-i.c.) | #5 (non-i.c.) | #6 (non- i-.c.) | #7(i.c.) | #8(i.c.) | #9(i.c.) | #10 (non-i.c.) | #11 (non-i.c.) | #12 (non-i.c.) |
| Blood | – | – | – | – | – | – | – | – | – | – | – | – |
| Oral swab | – | – | – | – | – | – | 35 dpi (37.75), 41 dpi (32.43) | 35 dpi (33.26), 40 dpi (30.85) | – | – | – | 56 dpi (36.58), 59 dpi (34.57) |
| Nasal swab | – | – | – | – | – | – | – | – | – | – | – | – |
| Rectal swab | – | – | – | – | – | – | – | – | – | – | – | |
| CSF | – | 56 dpi (33.49), 71 dpi (20.87) | 84 dpi (36.61) | – | – | – | 28 dpi (32.87), 41 dpi (29.04) | 28 dpi (18.25), 40 dpi (33.21) | 28 dpi (35.34), 35 dpi (33.83) | – | 28 dpi (39.89) | 56 dpi (36.35), 59 dpi (22.89) |
| Brain biopsy | n.d. | n.d. | 77 dpi (27.98) | – | – | – | n.d. | n.d. | n.d. | – | – | n.d. |
| Urine | 59 dpi (24.72) | |||||||||||
| (B) | Bornavirus-reactive antibodies | |||||||||||
| Animal ID | #1 (i.c.) | #2 (i.c.) | #3 (i.c.) | #4 (non-i.c.) | #5 (non-i.c.) | #6 (non- i-.c.) | #7 (i.c.) | #8 (i.c.) | #9 (i.c.) | #10 (non-i.c.) | #11 (non-i.c.) | #12 (non-i.c.) |
| Sera | 49 dpi (1:80); 56 dpi (1:640) | 70 dpi (1:320) | 63 dpi (1:160); 70 dpi (1:320); 77 dpi (1:640); 84 dpi (1:1280) | – | – | – | – | – | 35 dpi (1:80) | – | – | 56 dpi (1:80); 59 dpi (1:2560) |
| CSF | – | – | 84 dpi (1:40) | – | – | – | – | – | – | – | – | – |
(A) RT-qPCR results: blood and swabs were tested weekly and at necropsy, CSF monthly and at necropsy, urine only at necropsy, and brain biopsies were taken at day 77/78 postinfection. Cq values are given for time points with RT-qPCR positive results in brackets. (B) Serology results: sera samples were tested weekly and CSF monthly. Titres are given for time points with detectable antibodies.—= RT-qPCR negative or antibodies below the cut off (< 1:16) on all dates; n.d. = dot done. i.c. = intracerebral; and non-i.c. = peripheral route.