TABLE 1.

Details of primers used for immune‐related gene expression in this study

Gene	Oligo sequences	Annealing temperature (°C)	Product size (bp)	Gene functions	References
EF‐1α	F‐5′‐CTACAGCCAGGCTCGTTTCG‐3′ R‐5′‐CTTGTCACTGGTCTCCAGCA‐3′	56	139	Elongation factor (housekeeping gene)	Velázquez et al. (2018)
IgM	F‐5′‐GGATGACGAGGAAGCAGACT−3′ R‐5′‐CATCATCCCTTTGCCACTGG‐3′	53	122	Immunoglobulin M (IgM)	Velázquez et al. (2018)
IgT	F‐5′‐TGACCAGAAATGGCGAAGTCTG‐3′ R‐5′‐GTTATAGTCACATTCTTTAGAATTACC‐3′	53	163	Immunoglobulin T (IgT)	Velázquez et al. (2018)
IgD	F‐5′‐AACACCACCCTGTCCCTGAAT‐3′ R‐5′‐GGGTGAAAACCACATTCCAAC‐3′	61	127	Immunoglobulin D (IgD)	Wang et al. (2016)
CD4	F‐5′‐GCTCCAGTGTGACGTGAAA‐3′ R‐5′‐TACAGGTTTGAGTTGAGCTG‐3′	61	106	Receptor on helper T‐cell (CD4+)	XM_025911776.1, designed in this study
CD8	F‐5′‐GCTGGTAGCTCTGGCCTTT‐3′ R‐5′‐TGTGATGGTGTGGGCATCTC‐3′	49.5	91	Receptor on cytotoxic T‐cell (CD8+)	XM_005450353.3 a , designed in this study

^a Homolog (98% nucleotide sequence identity) of Oreochromis aureus CD8α (XM_031747820.2).