. 2021 Sep 3;44(12):2097–2109. doi: 10.1111/jfd.13523

TABLE 2.

Details of experimental groups and challenge results

Treatment	Administration route	No of fish challenged	Primary vaccination (TCID₅₀ fish⁻¹)	Booster vaccination (TCID₅₀ fish⁻¹)	Challenge (TCID₅₀ fish⁻¹)	Survival rate (%)	RPS (%)	Significant level (compared to control)
Treatment	Administration route	No of fish challenged	Day 0	Day 21	Day 28	Survival rate (%)	RPS (%)	Significant level (compared to control)
Control (L15 media)	IP	16 (× 2 rep.)	0	0	9 × 10⁵	28.13 ± 30.9	NA
HKV	IP	16 (× 2 rep.)	1.8 × 10⁶	1.8 × 10⁶	9 × 10⁵	81.3 ± 0.0	71.3	p < .0001
FKV	IP	15 (× 2 rep.)	1.8 × 10⁶	1.8 × 10⁶	9 × 10⁵	86.3 ± 0.0	79.6	p < .0001

FKV, formalin‐killed vaccine group; IP, intraperitoneal injection; HKV, heat‐killed vaccine group; NA, not applicable; rep, replicate.