Table 1.

RT-qPCR and qPCR assay oligonucleotides and calibration model performance metrics.

Viral target	Name	Sequence (5′ → 3′)	Y-intercept range	R2 range	Slope range	Amplification efficiency	References
Adenovirus	P: JTVXP	FAM-CTGGTGCAGTTCGCCCGTGCCA-TAMRA	40.254–42.307	0.990–0.997	−3.517 to −3.305	0.955 ± 0.027	(Jothikumar et al., 2005a)
	F: JTVXF	GGACGCCTCGGAGTACCTGAG
	R: JTVXR	ACIGTGGGGTTTCTGAACTTGTT
Norovirus GI	P: NV1LCpr	FAM-TGGACAGGAGAYCGCRATCT-TAMRA	37.987–39.580	0.990–0.998	− 3.427 to−3.279	0.981 ± 0.020	(da Silva et al., 2007; Svraka et al., 2007)
	F: QNIF4	CGCTGGATGCGNTTCCAT
	R: NV1LCR	CCTTAGACGCCATCATCATTTAC
Norovirus GII	P: QNIFS	FAM-AGCACGTGGGAGGGCGATCG-TAMRA	35.852–37.826	0.993–0.998	− 3.533 to −3.145	0.974 ± 0.044	(Loisy et al., 2005; Jothikumar et al., 2005b)
	F: QNIF2d	ATGTTCAGRTGGATGAGRTTCTCWGA
	R: COG2R	TCGACGCCATCTTCATTCACA
Hepatitis G	P: HepG-P	VIC-TCACCCATCCACCACCT-MGBNFQ	N/A	N/A	N/A	N/A	(Cashdollar et al., 2013)
	F: HepG-F	GCAAGCCCCAGAAACCG
	R: HepG-R	CAAGATGACCGGGATTTACGA
CrAssphage 056	P: 056P1	FAM-AATAACGATTTACGTGATGTAAC-MGBNFQ	40.452–42.307	0.987–0.996	−3.514 to − 3.397	0.951 ± 0.015	(Farkas et al., 2019)
	F: 056F1	CAGAAGTACAAACTCCTAAAAAACGTA
	R: 056R1	GATGACCAATAAACAAGCCATTA
Human Polyomavirus	P: KGJ3	FAM-TCATCACTGGCAAACAT-MG	38.645–40.63	0.985–0.99	−3.540 to 3.34	0.957 ± 0.027	(McQuaig et al., 2006)
	F: SM2	AGTCTTTAGGGTCTTCTACCTTT
	R: P6	GGTGCCAACCTATGGAACAG