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. Author manuscript; available in PMC: 2022 Aug 1.
Published in final edited form as: J Thorac Oncol. 2021 May 25;16(8):1333–1348. doi: 10.1016/j.jtho.2021.04.020

Figure 3. Obesity-mediated effects on progression of Lewis lung carcinoma tumors in mice.

Figure 3.

A. C57BL/6 mice were fed a high-fat (to induce obesity) or normal diet for ~14–16 weeks before s.c. implantation of 10×105 cells by s.c. injection into the shaved flanks. Mean tumor volumes were calculated using the formula: volume=0.5×lengthxwidth2. (B,C) Obese and normal mice were challenged with intravenous tail vein injection of 0.25×106 LLC-Luc cells. 26 days after injection, tumor burden (pulmonary metastases) were visualized by BLI after i.p. injection of d-luciferin (150mg/kg) using IVIS technology. P < 0.05 (*), < 0.02 (**), <0.001 (***) in standard t test. Error bars depict the SEM. Shown are a representative image (B) and mean tumor burden measurements (A and C) from 3–5 independent experiments.