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. 2021 Apr 22;111(2):367–377. doi: 10.1002/JLB.2A0520-334R

FIGURE 3.

FIGURE 3

Inhibition of downstream effector molecules Syk, PI3K and Rac GTPase reduces CD200R1L‐induced ROS production. (A,B) anti‐CD200R1L induced ROS production after pre‐incubation with small molecule inhibitors. (A) example experiment and (B) quantification of the area under the curve normalized to anti‐CD200R1L. Syk, PI3K, Akt: n = 9; Rac GTPase: n = 6 donors in 3 independent experiments. (C, D) example experiment and (D) quantification of the ROS production in anti‐CD200R1L‐stimulated neutrophils treated with PI3K isoform inhibitors normalized to anti‐CD200R1L. n = 7 donors in 3 independent experiments. A Wilcoxon signed‐rank test was used to compare conditions to vehicle control. *p < 0.05, **p < 0.01. Bars represent mean + SD