FIGURE 4.

CD200R1L stimulation induces NET release. (A) NETosis induced after 4 h stimulation with isotype control, anti‐CD200R1L or PMA analyzed by SYTOX‐green staining. Representative images for each stimulation are shown. Data are presented as total area NETing neutrophils (μm²). Mann‐Whitney test comparing anti‐CD200R1L or PMA stimulation with isotype control. (B) Purified healthy donor neutrophils were stimulated with IgG1 isotype control, anti‐CD200R1L, PMA with or without DPI for 4 h. Representative images for each stimulation are shown and data was analyzed as in Fig. 4A). Wilcoxon test. (A, B) Scale 100μ. Data from 8 donors in 4 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001