Figure 3.

Persistent activation of autophagy and endoplasmic reticulum stress in experimental chronic colitis are suppressed by chlorophyllin (CHL) treatment. A: representative images of p62/Sqstm1, LC3, LAMP1 expressed in the colon were determined by immunohistochemistry and positive signals were analyzed by ImageJ software. Three visual fields were counted for each mouse. Control n = 3 animals; DSS n = 4 animals; DSS+CHL n = 4 animals. The results shown are means ± SE. Statistical significance was determined by one-way ANOVA. *P < 0.05; ***P < 0.001. Scale bar = 100 μm. B: Western blot analysis of LC3 and p62 protein extracts of colon. C and D: expression of CHOP and Grp78 in the colon were determined by immunohistochemistry analysis and Western blot. DSS, dextran sulfate sodium.