Figure 6.

Effect of the lack of Lcn2 on muscle phenotype under mechanical unloading conditions. Eight‐week‐old WT and Lcn2 ^−/− mice were subjected to hindlimb suspension for 3 weeks. (a, b) Grip force was performed during the timeframe of the experiment in hindlimb suspended (HLS) or normal loading condition (NLC) (a) WT and (b)Lcn2 ^−/− mice. At sacrifice, serum was harvested to measure (c) CK serum activity. (d) Gastrocnemius, (e) quadriceps, (d) soleus, (g) EDL and (H) diaphragm muscles explanted from HLS and NLC mice were weighted and plotted as % of body weight. (a, b) Curve fitting test, N = 3–5. (c–h) Student's t test. *p < .05; ***p < .01; ***p < .001. CK, creatine kinase; EDL, Extensor Digitorum Longus; WT, wild‐type