Figure 7.

Effect of the lack of Lcn2 on muscle gene expression under mechanical unloading conditions. Eight‐week‐old WT and Lcn2 ^−/− mice were subjected to hindlimb suspension for 3 weeks. At sacrifice, quadriceps RNA was extracted, reverse‐transcribed and subjected to real‐time RT‐PCR to analyse the expression of (a) Lcn2, (b) Myod, (c) Myogenin, (d) Mef2C, (e) Pax7, (f) Desmin, (g) Utrophin, (H) Il6, (I) Il1b, and (J) Tnfa. Results are shown as fold to WT NLC. Student's t test. *p < .05. IL, interleukin; RT‐PCR, reverse transcrition polrmerase chain reaction