Confirmation of the presence of plasmid pKT230 in P. fluorescens LP59JG transformants. Lanes: A, smart ladder (Eurogentec); ND, nondigested profile of extracted plasmids; D, PstI-digested profile of extracted plasmids; 1, E. coli DH10B(pKT230); 2, clone from experiments with E. coli DH10B(pKT230) as donor in sterile soil; 3, clone from experiments with E. coli DH10B(pKT230) as donor in nonsterile soil; 4 and 5, clones from experiments with pKT230 as donor in sterile soil; 6, P. fluorescens LP59JG.