TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Resistance phenotypea | Use (reference) |
|---|---|---|
| Escherichia coli DH10B | Wild type | Donor cell |
| Pseudomonas fluorescens AK15 | Wild type | Donor cell (32) |
| Escherichia coli 1504 | Tetr (15) Spcr (60) | Recipient cell |
| Pseudomonas fluorescens LP59JG | Rifr (50) Gmr (10) | Recipient cell (11) |
| Agrobacterium tumefaciens GM19023 | Rifr (100) Smr (500) | Recipient cell |
| pKT230 (11.9 kb) | Kanr (50) Smr (100) | Transforming DNA (1) |
| pPZP111 (8.9 kb) | Kanr (25) Cmr (25) | Transforming DNA (13) |
a
Numbers in parentheses are antibiotic concentrations (in micrograms per milliliter).