TABLE 2.
Transformation rate and detection limit of experiments conducted in sterile soil microcosms
| Donor strain or plasmid | Recipient strain | Transformation ratea | Detection limit |
|---|---|---|---|
| E. coli DH10B (pKT230) | P. fluorescens LP59JG | 5.8 × 10−8 | 9.8 × 10−9 |
| pKT230 | P. fluorescens LP59JG | 8.3 × 10−8 | 2.0 × 10−8 |
| P. fluorescens AK15 (pPZP111) | A. tumefaciens GM19023 | 2.7 × 10−9 | 2.2 × 10−9 |
| pPZP111 | A. tumefaciens GMI9023 | 1.3 × 10−8 | 6.0 × 10−9 |
a
Efficiencies of transformation were expressed as the number of transformants relative to that of recipient cells.