TABLE 3.

Transformation rate and detection limit of experiments conducted in vitro

Donor strain or plasmid	Recipient strain	Transformation ratea	Detection limit
E. coli DH10B (pKT230)	E. coli 1504	<4.2 × 10−8	4.2 × 10−8
pKT230	E. coli 1504	<5.0 × 10−8	5.0 × 10−8
E. coli DH10B (pKT230)	P. fluorescens LP59JG	<1.3 × 10−7	1.3 × 10−7
pKT230	P. fluorescens LP59JG	<2.6 × 10−9	2.6 × 10−9
P. fluorescens AK15 (pPZP111)	A. tumefaciens GMI9023	NDb	4.4 × 10−9
pPZP111	A. tumefaciens GM19023	6.0 × 10−9	1.6 × 10−10

^aEfficiencies of transformation were expressed as the number of transformants relative to that of recipient cells. 

^bND, A. tumefaciens GM19023 was not detectable when cultivated concomitantly with P. fluorescens AK15 in vitro.