Table 2.

Activity of mono‐N‐aryl pentamidine analogues 30–36 against kinetoplastid parasites.

Entry	Compound	T. b. brucei				T. congolense				L. mexicana
		EC50 [μM; n=3]		RF	t‐test	EC50 [μM; n=3]		RF	t‐test	EC50 [μM; n=4]
		WT	B48			WT	DA‐Res
1	30	1.10±0.07	1.31±0.02	1.19	0.042	2.94±0.10	3.26±0.01	1.11	0.037	12.7±0.7
2	31	0.91±0.02	0.86±0.02	0.94	0.10	n.d.	n.d.	–	–	2.12±0.07
3	32	0.65±0.03	0.37±0.006	0.57	0.0007	1.26±0.03	1.56±0.06	1.23	0.012	9.71±0.56
4	33	0.19±0.003	0.28±0.006	1.47	0.0002	0.64±0.02	0.75±0.003	1.23	0.0008	0.66±0.04
5	34	0.51±0.004	0.51±0.02	1.01	0.82	n.d.	n.d.	–	–	>20
6	35	1.09±0.01	0.82±0.01	0.75	0.0001	n.d.	n.d.	–	–	>20
7	36	3.04±0.02	2.98±0.04	0.98	0.23	6.69±0.11	9.29±0.17	1.39	0.0002	7.54±0.59
8	PMD	0.0024±0.0002	0.28±0.002	116	3.5×10−8	1.31±0.02	1.42±0.01	1.08	0.013	2.04±0.04
9	DA	0.082±0.006	0.89±0.05	10.8	0.0001	0.27±0.008	1.71±0.02	6.4	2×10−7	n.d.

WT=wild‐type, PMD=pentamidine, DA=diminazene aceturate, DA‐Res=diminazene‐resistant cell line, RF=resistance factor being the ratio of EC₅₀ (resistant line) over EC₅₀(WT). Statistical difference between WT and resistant pairs of cell lines was established using a Student unpaired two‐tailed t‐test.