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. 2022 Jul 15;11(1):2101769. doi: 10.1080/2162402X.2022.2101769

Figure 2.

Figure 2.

MF-438 enhances the radiation sensitivity of both KYSE70 cells and KYSE410 cells. Fer-1 decreases the radiation sensitivity of tumor cells. (a) Colony formation assay was performed in KYSE70 cells following RT (0, 2, 4, 6, 8, 10, and 12 Gy), or RT/MF-438 treatment (0.25 μM). (b) Colony formation assay was performed in KYSE410 cells following RT (0, 2, 4, 6, 8, 10, and 12 Gy), or RT/MF-438 treatment (0.25 μM). (c) Dose-survival curves of KYSE70 cells infected with siRNAs targeting SCD1 were generated by MTT assay at 24 hours. (d) Dose-survival curves of KYSE410 cells infected with siRNAs targeting SCD1 were generated by MTT assay at 24 hours. (e) qRT-PCR of KYSE70 cells infected with siRNAs targeting SCD1. (f) qRT-PCR of KYSE410 cells infected with siRNAs targeting SCD1. (g) Colony formation assay was performed in KYSE70 cells infected with siRNAs targeting SCD1. (h) Colony formation assay was performed in KYSE410 cells infected with siRNAs targeting SCD1. (i) Colony formation assay was performed in KYSE70 cells following RT (0, 2, 4, 6, 8, 10, and 12 Gy), or RT/Fer-1 treatment (1 μM). (j) Colony formation assay was performed in KYSE410 cells following RT (0, 2, 4, 6, 8, 10, and 12 Gy), or RT/Fer-1 treatment (1 μM). Data are expressed as means ± SD (n = 3), n.s., non-significant, p > .05; *p ˂ 0.05; **p ˂ 0.01; ***p ˂ 0.001; ****p ˂ 0.0001.

Abbreviations: RT: radiation therapy; siNC: negative control siRNA; SCD1: stearoyl-coa desaturase.