Table 1.
Average plasmon wavelength (Ave pwl) of antibody−gold conjugates in various buffer conditions, with or without PEG2000 stabilization of conjugates, and at different levels of salt in the final reaction solution (NaCl). Standard Deviation (Std Dev) is based on 4 measurements.
| Buffer | PEG2000 | NaCl (mM) | Ave pwl | Std Dev | Condition chosen | Note |
|---|---|---|---|---|---|---|
| H6 | - | - | 574 ~ 600 | - | Unable to obtain stable measurement due to aggregation | |
| H6 | + | - | 544.2 | 6.4 | Measurable, yet broad and shallow peak | |
| H6 | + | 15 | 532.0 | 0.2 | Stable pwl profile, similar to that of PBS | |
| H6 | + | 30 | 532.0 | 0.2 | √ | H6N30 is chosen for high throughput assay |
| H6 | + | 40 | 528.7 | 0.2 | ||
| H6 | + | 50 | 528.7 | 0.1 | ||
| C6 | + | - | 530.0 | 0 | Upon PEG2000 treatment, C6 condition does not need additional salt for stabilization | |
| C6 | + | 30 | 529.0 | 0 | √ | C6N30 |
| A5 | + | 30 | 530.0 | 0 | √ | A5N30 |
| PBS | - | - | 530.0 | 0 | ||
| PBS | + | - | 530.0 | 0 | √ | PBS |