Table 2.
Human primer sequences used for qRT-PCR.
| Gene | Forward sequence 5’ – 3’ | Reverse sequence 5’ – 3’ |
|---|---|---|
| GAPDH | CAAATTCCATGGCACCGTCAA | AGCATCGCCCCACTTGATTT |
| β-actin | CGCGAGAAGATGACCCAGAT | AGCACAGCCTGGATAGCAAC |
| TNF-α | GAGGCCAAGCCCTGGTATG | CGGGCCGATTGATCTCAGC |
| IL-8 | ACTCCAAACCTTTCCACCCC | TTCTCAGCCCTCTTCAAAAACTTC |
| IL-6 | ACTCACCTCTTCAGAACGAATTG | CCATCTTTGGAAGGTTCAGGTTG |
| IL-1β | AGCTACGAATCTCCGACCAC | CGTTATCCCATGTGTCGAAGAA |
| IL-10 | TCAAGGCGCATGTGAACTCC | GATGTCAAACTCACTCATGGCT |
| IL-4 | ACAGCCTCACAGAGCAGAAGACT | TGTTCTTGGAGGCAGCAAAGA |
| IL-13 | CCTCATGGCGCTTTTGTTGAC | TCTGGTTCTGGGTGATGTTGA |
| IL-1Ra | CAATGCTGACTCAAAGGAGACGA | TCCCTCCATGGATTCCCAAGA |
| NF-κB (p50) | GCAGCACTACTTCTTGACCACC | TCTGCTCCTGAGCATTGACGTC |
| iNOS | GCCACAGAAGAGCCTGAGAG | GATCTCTGTGGGCGTGTGAT |
| MCP-1 | GAAAGTCTCTGCCGCCCTT | GGTGACTGGGGCATTGATTG |
| IP-10 | CTGTACGCTGTACCTGCATCA | TGATGGCCTTCGATTCTGGA |
| IL-15 | AACAGAAGCCAACTGGGTGAATG | CTCCAAGAGAAAGCACTTCATTGC |
| RIPK1 | AGCTCCTGGGCGTCATCATA | AGGTCTGCGATCTCGGCTTT |
| RIPK3 | TAATGTGGGCAGTGCTTGCT | GTCTGTCCTTGGGCTCACTG |
| BAD | CCCAGAGTTTGAGCCGAGTG | CCCATCCCTTCGTCGTCCT |
| BID | GGCCTACCCTAGAGACATGGA | AGACATCACGGAGCAAGGAC |
| Caspase 3 | CATACTCCACAGCACCTGGTT | TCAAGCTTGTCGGCATACTGT |
| Caspase 9 | TTTGAGGACCTTCGACCAGC | CGAAACAGCATTAGCGACCC |