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. 2022 Apr 22;21(15):1590–1598. doi: 10.1080/15384101.2022.2058839

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Figure 4. — PVT1 regulated ARPE-19 cell viability and apoptosis by upregulating KLF7. (a) The overexpression efficiency of KLF7 was verified by RT-qPCR. (B) The viability of ARPE-19 cells in HG+sh-NC, HG+sh-PVT1 and HG+sh-PVT1+ KLF7 groups was measured using MTT assay. (c-d) The ARPE-19 cell apoptosis in HG+sh-NC, HG+sh-PVT1 and HG+sh-PVT1+ KLF7 groups was detected by TUNEL assay. (e) The protein expression of ICAM, VEGF and TNF-α in HG+sh-NC, HG+sh-PVT1 and HG+sh-PVT1+ KLF7 groups was detected by western blotting assay. *p < 0.05, **p < 0.01, ***p < 0.001.