Figure 1.

De novo transcription of hras mRNA in reactive astrocytes is redox-sensitive. Relative mRNA expression levels by qPCR of hras and kras (A), iNOS and c-myc (B) genes in primary neocortical astrocytes challenged with 500 μM H₂O₂ for 30, 60 or 120 min in complete medium and in the presence or in the absence of Act D (10 μg/ml) pre-treatment. (C) qPCR analysis of astrocytic hras, kras, iNOS, c-myc treated with Act D alone as control (10 μg/ml; for 30, 60, 120 min). The mRNA levels are normalized to 18S RNA levels and the graph is plotted relative to untreated/growing value. Statistical analysis derived from four experiments in duplicate (n ≥ 8; mean ± SD); *P < 0.01 (matched pairs t-test) compared to untreated sample. See Supplementary Figure S1–S7 for statistical analysis raw data.