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. 2022 Jul 15;44(1):1169–1181. doi: 10.1080/0886022X.2022.2098764

Figure 3.

Figure 3.

Disulfiram can regulate pyroptosis through the caspase-11-GSDMD pathway in vivo. (a) Western blot analysis of GSDMD and N-GSDMD expression. (b) Western blot analysis of caspase-11 expression. (c) Western blot analysis of caspase-1 expression. (d) Western blot analyses of NLRP3 and ASC expression. (e) Western blot analysis of IL-1β and IL-18 expression. Protein expression was quantified by densitometric analysis. Each bar represents the mean ± SEM of three independent experiments. (f) Immunofluorescence analysis of the expression of caspase-11 and GSDMD in kidney tissues. Scale bar = 100 µm. *P < 0.05, **P < 0.01, ***P < 0.001 vs. sham; #P <.05, ###P < 0.001 vs. IR. ns, no significance.