Figure 4.

OE21 cells treated with Zap‐Pano and exposed to UV light have increased H3 K18 and H3 K9 acetylation. (A) OE21 cells were treated with the doses of Zap‐Pano indicated (0–10 μM) for 3 h and were then either kept in the dark (Con) for 24 h or treated with UV light for 5 min followed by incubation for 24 h. The levels of H3 K9 and H3 K18 acetylation are shown, H3 was included as a loading control. (B) OE21 cells were treated with 1 μM Zap‐Pano for 3 h followed by UV light (5 min) or no light (labelled C), the cells were allowed to grow for 5 h or 24 h before lysing and immunoblotting for H3K9Ac and H3K18Ac.