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Complementation of the Δasd deletion in P. putida by the cloned asd gene. The P. putida strains used were KT2440(pWW0) (circles), MCR7 (squares), Δasd(pWW0) (triangles), and MCR8 (diamonds). Cells were pregrown on M9 minimal medium with 3MB as the sole C source and supplemented with DAP, lysine, methionine, and threonine. Then cultures were diluted 50-fold in unsupplemented M9 minimal medium with glucose (A) (open symbols) or 3MB (B) (closed symbols) as the sole carbon source.
