TABLE 2.
The optimized mass spectrometry condition parameters in the determination of six monosaccharides were derivatized by 1‐phenyl‐3‐methyl‐5‐pyrazolone (PMP)
| MRM parameter | ||||||
|---|---|---|---|---|---|---|
| Component | t R/min | Molecular mass | Precursor ion | Product ion | CE/V | DP/V |
| D‐Galactose | 11.334 | 180.16 | 511.22 | 175.10/217.10 | 35 | 171 |
| D‐Arabinose | 12.534 | 150.13 | 481.21 | 175.10/217.10 | 33 | 163 |
| D‐Mannose | 5.050 | 180.16 | 511.22 | 175.10/217.10 | 35 | 171 |
| D‐Rhamnose | 6.713 | 164.16 | 495.22 | 175.10/217.10 | 33 | 174 |
| D‐Ribose | 6.363 | 150.13 | 481.21 | 175.10/217.11 | 33 | 163 |
| D‐Glucose | 9.956 | 180.16 | 511.22 | 175.10/217.10 | 35 | 171 |
Abbreviations: CE, collision voltage; DP, de‐cluster voltage; MRM, multiple reaction monitoring; t R, retention time