Nucleic acid extraction

2.5. When extracting nucleic acids for genomic testing, commercially available standardized kits suitable for use in clinical settings are preferred (C). If a kit is recommended for a specific genomic test, it should be used for the extraction (C, R).

Measurement of the purity and yield of nucleic acids

2.6. The purity and yield of the extracted nucleic acids should be determined with a spectrophotometer using the A260/A280 ratio, and using the fluorescence method or similar quantitation methods (C, R).

Assessment of nucleic acid quality

2.7. If the nucleic acids extracted from the FFPE samples have been stored for a long period, or if deterioration of nucleic acid quality is suspected due to over fixation, it is recommended that the quality of the nucleic acids should be assessed (C, R).