FIGURE 2.

Increased lipogenesis is critical for self‐renewal and growth of breast cancer stem cells. A,B, Representative cell morphology of MCF‐7‐CSC (A) and EMT‐CSC (B) were shown the inhibition of cell growth following the indicated treatments for 48 hours. C, Quantitation of sphere formation in (A). D, Quantitation of cell viability in (B). E,F, Representative image of immunoblotting and relative densiometric bar graphs of SCD1 expression in MCF‐7‐CSC (E) and EMT‐CSC (F). G,H, Decreased cell viability was observed in MCF‐7‐CSC (G) and EMT‐CSC (H) after the treatment of SCD1‐specific siRNA. ***P < .001 vs vehicle‐treated control cells. All the experiments were repeated at least three times. CSC, cancer stem cells; EMT, epithelial‐mesenchymal transition; siRNA, small interfering RNA