FIGURE 4.

n‐3 PUFA suppress the growth of breast cancer stem cells by blocking lipid metabolism in vitro. A,B, Representative cell morphology of MCF‐7‐CSC (A) and EMT‐CSC (B) treated with 20 μM of indicated fatty acids for 48 hours. C, Quantitation of sphere formation in (A). D, Quantitation of cell viability in (B). E,F, Representative Western blot for protein expressions of the lipogenic enzymes in MCF‐7‐CSC (E) and EMT‐CSC (F) treated with indicated fatty acids. G,H, mRNA expressions of the lipogenic enzymes in MCF‐7‐CSC (G) and EMT‐CSC (H) after the treatment of indicated fatty acids. *P < .05; **P < .01; ***P < .001 vs either vehicle‐treated control cells or their corresponding parental cells. All the experiments were repeated at least three times. CSC, cancer stem cells; EMT, epithelial‐mesenchymal transition; PUFA, polyunsaturated fatty acids