FIGURE 5.
n‐3 PUFA inhibit tumor growth of breast cancer stem cells in xenograft mouse model. A, Representative image of tumor‐bearing mice. Prior to implanting 1.5 × 107 of MCF‐7‐CSC to nude mice, these mice were orally gavaged by either fish oil (n‐3 PUFA) or corn oil (control) for a month and continued giving oils until the end of the experiment. B, Tumor growth of the nude mice in each group. C, Relative tumor weight by body weight. D, The ratio of n‐6/n‐3 PUFA. E, The ratio of MUFA (C16:1 + C18:1)/SFA (C16:0 + C18:0). F, The ratio of C20:4/C18:2. G,H, Protein levels (G) and mRNA levels (H) of SCD1, FAS, FADS2, and COX2 between control and n‐3 PUFA groups. n = 3 to 4. *P < .05; **P < .01; ***P < .001 vs control. MUFA, monounsaturated fatty acids; PUFA, polyunsaturated fatty acids; SFA, saturated fatty acids