Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Diagram of the mutation site at the junction between the K2S and gpIII genes on pComb3H-K2S. The annealing site of pComb3H-K2S was bound with a set of mutation primers (MSTPA and MASTPA) containing modified oligonucleotides (underlined). After the cycle amplification was performed, the SfiI site 1 (in boldface) was modified and lost in the newly synthesized strand.
