TABLE 1.
Detection of rK2S molecule in phage preparation by sandwich ELISA
| Capture antibody | Value for tracer antibody (conjugated with HRP)c
|
|||
|---|---|---|---|---|
| Anti-tPA
|
Anti-M13
|
|||
| K2S-φ | VCSM13a | K2S-φ | VCSM13a | |
| Anti-kringle 2b | 1.12 ± 0.04 | 0.12 ± 0.03 | 1.89 ± 0.02 | 0.16 ± 0.02 |
| Anti-M13 | 0.17 ± 0.01 | 0.14 ± 0.05 | 1.91 ± 0.02 | 1.88 ± 0.03 |
a
VCSM13 was harvested from XL-1 Blue transformed with pComb3HSS.
b
Mouse monoclonal anti-kringle 2 (16/B) antibody was used. The other antibodies were prepared from sheep immunoglobulin.
c
Data are the absorbance values (means ± standard deviations) of samples assayed in triplicate.