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. 2021 Aug 19;51(10):2513–2521. doi: 10.1002/eji.202049107

Figure 4.

Figure 4

Constitutive expression of the mBE and mBG fusion proteins prevented allogeneic NK cell‐mediated lysis and recognition by allogeneic T cells. (A) Allogeneic NK cell cytotoxicity on DKO gene‐edited CAR T‐19 cells after coculture at an NK‐to‐CAR T cell ratio of 10:1 for 4 h (n = 4 different donors in four independent experiments. **P < 0.01, ***P < 0.001, versus DKO‐CAR T‐19, t‐test, GraphPad Prism version 5). (B) Recognition by allogeneic T cells. Allogeneic PBMCs cocultured with mitomycin C‐pretreated CAR T‐19 and DKO gene‐edited CAR T‐19 cells at a PBMC‐to‐CAR T‐19 cell ratio of 5:1 and the number of CD3+ cells were calculated for 48 and 72 h (n = 3 different donors in three independent experiments. *P < 0.05, CAR T‐19 versus DKO‐CAR T‐19, DKO‐CAR T‐19mBE or DKO‐CAR T‐19mBG, t‐test, GraphPad Prism version 5). (C) Specificity of DKO gene‐edited CAR T‐19 cells. Allogeneic PBMCs cocultured with CAR T‐19 and DKO gene‐edited CAR T‐19 cells at a PBMC‐to‐CAR T‐19 cell ratio of 5:1 and the number of CD19+ cells were calculated for 48 and 72 h (n = 3 different donors in three independent experiments).