TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| Pseudomonas sp. strain Cam-1 | Wild type | 34 |
| Pseudomonas sp. strain Cam-10 | bphA-lacZ-Gmr | This study |
| Pseudomonas sp. strain Cam-20 | bphA-xylE-Gmr | This study |
| Burkholderia sp. strain LB400 | Wild type | 10 |
| Burkholderia sp. strain LB400-1 | bphA-lacZ-Gmr | This study |
| Escherichia coli XL1-Blue MR | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac | Stratagene |
| Escherichia coli DH5α | endA1 hsdR17(rk− mk−) supE44 thi-1 recA1 gyrA(Nalr) relA1 Δ(lacZYA-argF) U169 deoR[φ80dlac (lacZ)M15] | Gibco BRL |
| Escherichia coli S17-1 | recA pro thi hsdR with integrated RP4-2-tc::Mu-kan::Tn7; Tra+ Trr Smr | 44 |
| Plasmids | ||
| pUC19 | Cloning vector; Ampr | 49 |
| pT7-7 | Cloning vector; T7 promoter; Ampr | 46 |
| pT7-6a | bphAEFG from Burkholderia sp. strain LB400 inserted into multiple cloning site of pT7-6 | 30 |
| pEM1 | SuperCosl cosmid library clone containing bphAEFGBC gene cluster from Cam-1 | This study |
| pEM10 | bphAEFGBC containing 8-kb SacI fragment from pEM1 inserted into SacI site of pUC19 | This study |
| pT7-7a | 4-kb BamHI fragment PCR amplified from pEM10 inserted into BamHI site of pT7-7 | This study |
| pEX100T | sacB conjugable plasmid for gene replacement; Ampr | 43 |
| pX1918GT | Plasmid containing the xylE-gentamicin resistance cassette; Ampr Gmr | 43 |
| pUCGm | Plasmid containing the gentamicin resistance cassette; Ampr Gmr | 42 |
| pIND/lacZ | Plasmid containing lacZ, Ampr, Neor | Invitrogen |
| pEM2 | 4-kb BamHI fragment from pT7-7a inserted into SmaI site of pEX100T | This study |
| pEM20 | 877-bp XbaI fragment from pUCGm inserted into XbaI site of pIND/lacZ | This study |
| pEM21 | 4-kb PmeI fragment from pEM20 inserted into EcoRI site of pEM2 | This study |