Table 1.
Primers used in qPCR assay
| Genes | Primer | Primer sequence（5′‐3′） | Product |
|---|---|---|---|
| PKD1 | Forward | TGGGTGCTGATGTTCGTAGA | 134 bp |
| Reverse | CGTAATGCAAATGGGCGGAC | ||
| Sirt1 | Forward | GATCTCCCAGATCCTCAAGCC | 189 bp |
| Reverse | TTCCACTGCACAGGCACATA | ||
| c‐MYC | Forward | ACTCGGTGCAGCCCTATTTC | 187 bp |
| Reverse | GTAGCGACCGCAACATAGGA | ||
| RUNX2 | Forward | GCGGTGCAAACTTTCTCCAG | 196 bp |
| Reverse | GTCACTGCACTGAAGAGGCT | ||
| COL1A1 | Forward | TTTCCCCCAACCCTGGAAAC | 287 bp |
| Reverse | CAGTGGGCAGAAAGGGACTT | ||
| BGLAP | Forward | TGAGGACCCTCTCTCTGCTC | 150 bp |
| Reverse | GGTAGCGCCGGAGTCTATTC |
Abbreviation: BGLAP, bone gammacarboxyglutamate (gla) protein (osteocalcin); qPCR, quantitative polymerase chain reaction.