FIG. 2.

Distribution of carbon at the pyruvate branch point as a function of aeration. As an example, the in vivo specific activity of PFL at steady state 3 was computed as follows: r_PFL = concentration of formate × dilution rate/biomass concentration = 26.25 (C-mmol · liter⁻¹) × 0.1 (h⁻¹)/0.58 (g [dry weight] · liter⁻¹ = 4.77 C-mmol · g (dry weight)⁻¹ · h⁻¹. The in vivo specific activity of PDH was computed as r_PDH = [(r_{acetate kinase} + r_ADH)/2] − r_PFL in millimoles of carbon per gram (dry weight) per hour.