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. 2001 Jun;67(6):2677–2682. doi: 10.1128/AEM.67.6.2677-2682.2001

FIG. 3.

FIG. 3

Synthesis of NAD+ by ADH, LDH, NOX, and PDH as function of growth conditions. As an example, the in vivo specific activity through ADH for steady state 2 was calculated as follows: rADH = (concentration of ethanol) × (moles of NAD+ generated per C-mole of ethanol generated) × (dilution rate)/(biomass concentration) = 15.92 (C-mmol · liter−1) × 1 (mmol of NAD+ · C-mmol−1) × 0.1 (h−1)/0.60 (g [dry weight] · liter−1) = 2.65 mmol of NAD+ · g (dry weight)−1 · h−1. The in vivo flux through NOX was calculated as rNOX = (number of moles of NAD+ generated per mole of O2 consumed) × (specific uptake rate of oxygen).