TABLE 1.
Steady state | N2/air ratio | Biomass concn (g [cell dry wt] · liter−1) | Yield of biomass on glucose (g [cell dry wt] · g of glucose−1) | Specific uptake rate of glucose (C-mmol · g [cell dry wt]−1 · h−1) | Specific uptake rate of oxygen (mmol of O2 · g [cell dry wt]−1 · h−1) |
---|---|---|---|---|---|
1 | 250:0 | 0.54 ± 0.02 | 0.18 | 18.4 | |
2 | 230:20 | 0.60 ± 0.02 | 0.20 | 16.7 | 0.52 |
3 | 200:50 | 0.55 ± 0.02 | 0.18 | 18.2 | 1.39 |
4 | 125:125 | 0.69 ± 0.04 | 0.22 | 14.4 | 2.83 |
5 | 80:170b | 0.68 ± 0.01 | 0.22 | 14.7 | 3.61 |
Total gas flow was 250 N ml · min−1. In steady state 5, DOT was controlled at 5% (13 μmol of O2 · liter−1).
Approximate ratio.