Figure 3.
Pollen viability and Fe content in floral buds of plants grown in Fe‐deficient medium.
(a–d) Alexander staining of mature pollen from plants grown in Fe‐sufficient (+Fe, a and b) and Fe‐deficient (−Fe, c and d) hydroponic medium and treated without (a, b) and with 50 μm Na2‐EDTA (Mock, c) or 50 μm NaFe‐EDTA (Fe, d) by dropping the solution on floral buds. (e) Percentage of viable pollen calculated from Alexander staining of pollen. (f) Fe concentration of floral buds. Data are shown as mean ± standard error of three independent experiments. **P < 0.01, ***P < 0.001 compared with Fe‐sufficient plants (Student’s t‐test). Bars = 50 μm in (a–d).