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. 2001 Jun;67(6):2683–2691. doi: 10.1128/AEM.67.6.2683-2691.2001

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Copyright © 2001, American Society for Microbiology

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FIG. 2 — Southern hybridization of selected isolates with the nah genes for naphthalene degradation from P. putida NCIB 9816-4. EcoRI-digested genomic DNA was probed with a 3.5-kb SalI fragment from P. putida NCIB 9816-4 containing the genes for naphthalene dioxygenase. M1 and M2 are the digoxigenin-labeled and high-molecular-weight markers, respectively. Lanes 1 to 4 are the pseudomonad isolates PR-N7, PR-N9, PR-N10, and PS-P2; lane 5 is the sphingomonad isolate PR-P12; lanes 6 and 7 are the nocardioform isolates PR-N14 and PR-N15; lane 8 is Arthrobacter sp. strain PR-P3; and lanes 9 and 10 represent the mucoid and nonmucoid Paenibacillus isolates PR-N5 and PR-P1, respectively. P is the positive control P. putida NCIB 9816-4, and N1 and N2 are the negative controls C. testosteroni GZ39 and S. yanoikuyae B1, respectively.