FIGURE 1.

Curcuminoids downregulated HER2 protein expression in HER2‐overexpressing bladder cancer cells. RT4 and TCCSUP cells were treated with various concentrations of (A) curcumin (Cur), (B) bisdemethoxycurcumin (BDMC), and (C) demethoxycurcumin (DMC), as indicated. Cell viability was determined by MTT assays. (D) RT4 cells were treated with vehicle (DMSO), indicated concentrations of Cur, BDMC, and DMC for 48 h followed by Western blot for HER2, and β‐actin. Representative of two separate experiments. Western blot data presented are representative of those obtained in at least three separate experiments. The values below the figures represent change in protein expression of the bands normalized to β‐actin. (E) RT4 cells were grown on coverslips and treated with vehicle (DMSO), indicated concentrations of DMC for 48 h, then subjected to immunofluorescent staining with HER2 and DAPI as described in materials and methods. Fluorescence images were performed by fluorescent microscopy