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. 2022 Jul 4;13:931021. doi: 10.3389/fimmu.2022.931021

Figure 5.

Figure 5

TLR22-induced mitochondrial fragmentation dissipates ψm and triggers Cyt c release actvating caspase-9/caspase-3 axis in A. hydrophila-infected HKM. HKM pre-incubated with CsA, Mdivi-1 or transfected with sc-siRNA, tlr22-siRNA were infected with A. hydrophila and at 24 h p.i., (A) changes in ψm were studied using Rhodamine-123, and (B) changes in Cyt c release was studied. Data are presented as box-and-whisker plots (n=5), shows the median and 25th and 75th percentiles, and the whiskers show 10th and 90th percentiles. Vertical bars denote mean ± SEM (n=5). HKM pre-incubated with CsA, Mdivi-1, Z0LEHD-FMK, Ac-DEVD-CHO or transfected with sc-siRNA, tlr22-siRNA were infected with A. hydrophila and at 24 h p.i., (C) caspase-9 activity and (D) caspase-3 activity were studied. Vertical bars denote mean ± SEM (n=3). Asterisk (*) signifies significant difference between the indicated group (*p < 0.05). HKM, uninfected HKM; HKM+B, HKM infected with A. hydrophila; HKM+CsA+B, HKM+Mdivi-1 +B, HKM+Z-LEHD-FMK, HKM+Ac-DEVD-CHO; HKM pre-incubated with CsA, Mdivi-1, Z-LEHD-FMK, Ac-DEVD-CHO were infected with A. hydrophila; HKM+sc-siRNA, sc-siRNA transfected HKM; HKM+sc-siRNA+B, HKM+tlr22-siRNA+B, HKM+chop-siRNA+B, sc-siRNA, tlr22-siRNA and chop-siRNA transfected HKM were infected with A. hydrophila.