Fig. 5.

Camalexin accumulation after egg extract and Botrytis cinerea BMM treatment. Plant genotypes were pretreated with Pieris brassicae egg extract (EE) for 5 d, and distal leaves were further treated with a mock solution or infected with B. cinerea. Untreated plants were used as controls (CTL). (a,b) Camalexin concentrations were measured in distal leaves. Means ± SE of three independent experiments are shown (n = 10–12 per experiment). For each time point, different letters indicate significant differences at P < 0.05 (ANOVA followed by Tukey’s Honest Significant Difference test). (c) In vitro growth inhibition assay. Radial growth of a B. cinerea colony growing on potato dextrose agar plates supplemented with different concentrations of camalexin or indole‐3‐carboxylic acid (ICA) was measured after 24 h of incubation. Solid line, mean; shaded band, SE (n = 12). This experiment was repeated twice with similar results.