FIGURE 2.

Technical considerations in Hi‐C scaffolding. The major points regarding technical considerations (left) are shown as hands‐on steps. Individual rows show possible solutions (middle) and our demonstration using the Madagascar ground gecko (right). See Naumova et al. (2013) for the detail of the potential effect of cell cycle status to chromatin contacts. See Kadota et al. (2020) for the method to estimate the optimal number of PCR cycles for library amplification