FIGURE 3.

The role of dendritic cell (DC)‐released extracellular vesicles (DCEVs) in house dust mite (HDM)‐induced allergic airway inflammation. Mice were sensitized with different stimuli‐induced DCEVs (HDM‐DCEVs/HDM group, (HDM + hemin)‐DCEVs/HDM group, hemin‐DCEVs/HDM group), and challenged with HDM. HDM or PBS‐sensitized and challenged mice were as a control group (HDM/HDM, PBS/PBS). A: illustration of the model; B: the pathologic changes of mouse mediastinal lymph nodes (MLNs) were observed using H&E staining; C: the pathological changes of mouse lung tissue were observed using H&E staining; and D: the levels of cytokines in lung tissue homogenate were detected with ELISA. For analysis of ELISA, data are pooled from three independent experiments with three mice each group. The data are shown as mean ± sd. Data for compared with the PBS/PBS group, *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. Data for compared with the HDM/HDM group, # p < 0.05; ### p < 0.001; i.n., intranasally