FIGURE 4.

Hemin‐treated dendritic cell (DC)‐released extracellular vesicles (DCEVs) alleviate house dust mite (HDM)‐induced allergic airway inflammation. HDM‐sensitized and challenged mice were instilled intranasally with 100 μg hemin‐DCEVs 1 d before sensitization and challenge (HDM + hemin‐DCEVs group). A: illustration of the model; B: representative images of proximal airway showing inflamed lung areas; C: the levels of IL‐4/IL‐5/IL‐13/IL‐17‐A/IFN‐γ/IL‐10 in lung homogenates; and D: flow cytometry (FCM) of eosinophils (CD45⁺ Siglec‐F⁺ CD11c⁻) and neutrophils (CD45⁺ Ly6G⁺ CD11b⁺) in bronchoalveolar lavage fluid (BALF). For analysis of ELISA and FCM, data are pooled from three independent experiments with three mice each group. The data are shown as mean ± sd, *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant; i.n., intranasally