FIGURE 2.

A bone cancer pain (BCP) model was successfully established. (a) The mechanical pain threshold of the ipsidal hindfoot in rats with BCP decreased from day 6 to day 18 after surgery (***p< 0.001 vs. sham group; n = 6, two‐way repeated‐measures ANOVA). (b) Three‐dimensional (3D) CT reconstruction showed significant cortical bone destruction after tumour inoculation. Arrowhead points to the spot where tumour cells caused abnormal bone structure. n = 10. (c) (1, 2) Haematoxylin–eosin staining showed that the bone marrow cavity of rats in the sham group was filled with lymphocytes, red blood cells and macrophages (yellow triangle). Regular arrangement of trabecular bone (asterisks) can also be seen in the bone marrow cavity. (3, 4) After tumour inoculation, cancer cells (within the dotted lines) and bone resorption pits (red arrows) appeared in the bone marrow cavity of rats with BCP. Figures c(2, 4)) are high‐magnification images of the selected area with white frames; n = 10. Scale bars: 200 μm (top row), 50 μm (bottom row). BL, baseline. n represents the number of experimental animals in each group